High-level expression of soluble recombinant RNase P protein from Escherichia coli.
نویسندگان
چکیده
We have expressed recombinant RNase P protein from Escherichia coli in high yield. A hexahistidine sequence at the amino terminus allowed protein purification in a single step. Mass spectrometry confirmed the molecular weight of the purified protein and indicated a purity of > 95%. Protein functionality was demonstrated by reconstitution of active holoenzyme.
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ورودعنوان ژورنال:
- Journal of bacteriology
دوره 177 9 شماره
صفحات -
تاریخ انتشار 1995